ABSTRACT
<p><b>OBJECTIVES</b>To study the effects of Sanqi qisodium hyaluronate gel on collagen-I and collagen-III expression in the process of rabbits' epidural scar formation after operation.</p><p><b>METHODS</b>Ninety-six white rabbits with 6-month-old, half males and half females, weighted from 2 to 2.5 kg, which were randomly divided into normal saline group (A), Sanqi group (B), qisodium hyaluronate group (C) and Sanqi qisodium hyaluronate gel group (D). The laminectomy of rabbits were performed in group A, B, C, D, the duras were surrounded with normal saline, Sanqi liquid, qisodium hyaluronate and Sanqi qisodium hyaluronate gel respectively. Animals of each group were killed at 1, 2, 4, 8 weeks after operation. Use Masson staining for histological observation of collagen, and in situ hybridization staining for the analysis of collagen-I and collagen-III expression.</p><p><b>RESULTS</b>In the Masson staining, Sanqi qisodium hyaluronate gel group was more regular than the control group in the shape of collagen texture. As to the expression of collagen-I, and Sanqi qisodium hyaluronate gel group was lower than normal saline group, the Sanqi group and qisodium hyaluronate group at 4 weeks after using medicine (P < 0.01); while the Sanqi qisodium hyaluronate gel group was higher than normal saline group, Sanqi group and qisodium hyaluronate group in the collagen-III expression (P < 0.01).</p><p><b>CONCLUSIONS</b>Sanqi qisodium hyaluronate gel could improve collagen's arrangement of the rabbit's epidural scar after operation, reduce its rigidity and increase flexibility.</p>
Subject(s)
Animals , Female , Male , Rabbits , Cicatrix , Metabolism , Collagen Type I , Genetics , Metabolism , Collagen Type III , Genetics , Metabolism , Epidural Space , Gels , Gene Expression Regulation , Hyaluronic Acid , Chemistry , Laminectomy , RNA, Messenger , Genetics , MetabolismABSTRACT
Transforming growth factor (TGF-beta) plays a central role in wound healing and scarring. TGF-beta is a member of cytokine family, involving cell proliferation, migration, differentiation and apoptosis, which subsequently trigger extracellular matrix (ECM) deposite and collagen overproduction. A number of regulators of TGF-beta expression bave been identified, including Decorin CTGF, etc. TCM can reduced later scarring, and it accelerates wound healing. TCM can reduce the expression of TGF-beta, resistance of fibrosis, so that lighten the development of scar. This article reviewed the mechanism of TGF-beta in the scarring and TCM can decrease the development of scar effected by TGF-beta.